🧪 Exciting News! We’ve launched our New Lab Program - empowering innovation with cutting-edge research facilities. <a href="https://cellculturecollective.com/new-lab-program/" target="_blank">Explore Now! 🔬</a>
🧪 Exciting News! We’ve launched our New Lab Program - empowering innovation with cutting-edge research facilities. <a href="https://cellculturecollective.com/new-lab-program/" target="_blank">Explore Now! 🔬</a>
$24.00
The IGFs, or insulin-like growth factors, are polypeptide growth factors known for their ability to stimulate the proliferation and survival of various cell types, including those found in muscle, bone, and cartilage tissue when studied in vitro. While primarily synthesized in the liver, different tissues produce IGFs at specific times. Belonging to the Insulin gene family, which also includes insulin and relaxin, IGFs share structural and functional similarities with insulin but exhibit significantly greater growth-promoting activity.
IGF-II expression is influenced by placenta lactogen, while growth hormone regulates IGF-I expression. Both IGF-I and IGF-II signal through the tyrosine kinase type I receptor (IGF-IR), though IGF-II can also utilize the IGF-II/Mannose-6-phosphate receptor pathway. Mature IGFs are formed through proteolytic processing of inactive precursor proteins, which contain N-terminal and C-terminal propeptide regions.
Recombinant Human IGF-I and IGF-II are globular proteins composed of 70 and 67 amino acids, respectively, and each contains three intra-molecular disulfide bonds. IGF-I LR3, a recombinant analog of Human IGF-I, features the complete IGF-I sequence with an Arginine substitution for the third position Glutamic acid, as well as a 13-amino acid N terminus peptide extension. Engineered for enhanced biological potency in vitro, IGF-I LR3 boasts an extended half-life and reduced binding affinity to native proteins within the body, making it well-suited for both research purposes and large-scale culturing. Recombinant Human IGF-I LR3 is a single, non-glycosylated polypeptide chain weighing 9.1 kDa and consisting of 83 amino acid residues.
Product Specifications
Species Human
Published Species Human
Expression System E.coli
Amino Acid Sequence MFPAMPLSSL FVNGPRTLCG AELVDALQFV CGDRGFYFNK PTGYGSSSRR APQTGIVDEC CFRSCDLRRL EMYCAPLKPA KSA
Molecular Weight 9.1 kDa
Class Recombinant
Type Protein
Purity ≥ 98% by SDS-PAGE gel and HPLC analyses.
Endotoxin Concentration <0.1 EU/µg
Activity The ED50 was determined by a cell proliferation assay using FDC-P1 cells is ≤ 2.0 ng/ml, corresponding to a specific activity of ≥ 5 x 105 units/mg.
Conjugate Unconjugated
Form Lyophilized
Contains No Preservative
Storage Conditions -20°C
Long before the double helix became a symbol of modern biology, its shape remained an enigma. Scientists suspected DNA held the key to life’s code, but its structure was still out of reach. In a lab at King’s College London, Rosalind Franklin began a line of experiments that would change
Every lab experiment carries more than just scientific weight. It carries the pressure of deadlines, grant expectations, publication goals, and the simple but critical need for results that can be trusted. Yet all too often, experiments are compromised not because of a flawed hypothesis, but because of something much smaller;
Behind every successful experiment is a series of small, consistent choices and often, the most overlooked are the tools we use every day. In cell culture, even the best protocols and techniques can’t compensate for consumables that fall short on sterility, reliability, or performance. Contamination, variability, and unexpected failures rarely
In the intricate world of cell culture, precision is everything. Monitoring plays a pivotal role in ensuring that cells thrive, experiments succeed, and data remains reliable. However, for many researchers, this critical aspect often becomes a source of frustration. From contamination to data inconsistencies, the challenges of cell culture monitoring
A future where new drugs are developed faster, safer, and with greater precision, without relying on animal testing or lengthy clinical trials. A future where treatments are tailored specifically to your genetic makeup, providing maximum efficacy with minimal side effects. This is the promise of 3D bioprinting in drug discovery,
Imagine a future where your favorite burger doesn’t come from a farm but a lab. It is produced without slaughter, with minimal environmental impact, and with the same taste and texture you know and love. This is the promise of cell-based meat cultivation, a revolutionary approach that’s reshaping the way
If you’re working with cell cultures, you already know how critical it is to monitor cell viability and growth at every stage. Whether you’re a PhD student in the middle of a crucial experiment, a seasoned scientist in biotech, or part of a cutting-edge research lab, keeping a close eye
The field of cell culture is evolving at an unprecedented pace, driving breakthroughs across biomedical research, drug discovery, and sustainable bioproducts. From 3D models revolutionizing cancer research to bioreactors scaling up production, the innovations in this space are transforming how we approach science and medicine. In 2022, the global cell
Scaling up cell cultures is an art, one that bridges the gap between routine lab work and groundbreaking discoveries. Whether you’re cultivating cells for life-saving therapies, high-precision drug testing, or cutting-edge biomanufacturing, the challenge lies in achieving consistent growth while safeguarding cell viability and functionality. Efficient cell culture expansion isn’t
