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🧪 Exciting News! We’ve launched our New Lab Program - empowering innovation with cutting-edge research facilities. <a href="https://cellculturecollective.com/new-lab-program/" target="_blank">Explore Now! 🔬</a>
$90.00
Defined Bioscience’s FGF2-G3 (bovine), or btFGF2-G3, is a thermostabilized growth factor that offers a novel way to grow FGF2-dependent cell cultures more efficiently, with fewer medium changes. The stability of FGF2-G3 in culture allows for a more homogeneous, undifferentiated stem cell culture, and daily medium change is not required. Contact us for larger quantities to get a quote.
btFGF2-G3 has an expected EC50 of less than 1 ng/mL, as determined in-house for all lots by an NIH-3T3 cell proliferation assay.
btFGF2-G3 differs from the native sequence of bovine FGF2 (UniProt accession P03969) at nine key residues found to promote increased thermostability and function in cell culture for human FGF2. FGF2-G3 is critical for use in cell culture conditions greater than room temperature; and helps to eliminate the need for weekend supplementation and daily medium changes for stem cell culture.
btFGF2-G3 is supplied as a 1 mg/mL solution in storage buffer or in a freeze-dried form that can be reconstituted into most standard buffer formulations as needed for downstream use. btFGF2-G3 can be substituted directly for native FGF2, with dosage testing recommended for optimal performance. It is recommended to reconstitute powder btFGF2-G3 to 1 mg/mL with water before use.
1- D. Pavel, D. Bednar, P. Vanacek, L. Balek, L. Eiselleova, V. Stepankova, E. Sebestova, M. Kunova Bosakova,
Z. Konecna, S. Mazurenko. A. Kunka, T. Vanova, K. Zoufalova, R. Chaloupkova, J. Brezovsky, P. Krejci, Z.
Prokop, P. Dvorak, J. Damborsky, Biotechnology and Bioengineering 2018, 115, 850.
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