Defined Bioscience’s DMEM/F12 is a fully defined basal medium for use in the culture of a wide range of mammalian cells. DMEM/F12 is a 1:1 mixture of DMEM and Ham’s F-12, providing an optimized environment for a broad range of cell types. Our DMEM/F12 is buffered with HEPES and sodium bicarbonate, offering a comprehensive buffering system which helps to maintain pH stability and minimize the effect of CO2 fluctuations in standard incubator conditions for mammalian cell culture. Additionally, this formulation includes L-alanyl-L-glutamine in place of glutamine, which enhances cell metabolism and reduces the risk of cell death, while also improving medium stability and cell culture consistency.