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Defined Bioscience’s HiDef™ PBS/EDTA is a defined reagent for protein-free adherent cell dissociation, for routine aggregate passaging or single cell suspension. HiDef PBS/EDTA is designed to detach cells from surfaces without impacting cell functionality or surface protein integrity, with applications in stem, tumor, and primary cell culture, among others. HiDef PBS/EDTA can also be used as a cell washing step prior to detachment using other methods, including enzymatic digestion (e.g. trypsinization).
HiDef PBS/EDTA is provided as a 1x solution, 125 mL per bottle, and consists of Dulbecco’s Phosphate Buffered Saline (DPBS, without Ca2+ and Mg2+) pH 7.4 with 0.5 mM EDTA. Each lot of HiDef PBS/EDTA is used in combination with HiDef-B8 medium in performance testing in a culture assay evaluating using human iPSCs to confirm appropriate and consistent detachment.
HiDef™ PBS/EDTA is provided at 1x in liquid form in 125-mL volumes. This supplement can be used with cells cultured in a wide range of medium formulations, including Defined Bioscience’s fully defined HiDef-B8 stem cell medium (Catalog #LSS-204).
Each lot of HiDef PBS/EDTA is used in performance testing in a culture assay evaluating cell detachment.
Fully Chemically Defined- Serum and Animal Component Free
This product is shipped on dry ice
Store at -20C to -80C until ready for use
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With over 20 years of experience in the life science industry, we work hard to bring our customers the highest quality and most relevant products and services. We specialize in cell culture and diagnostic processed serum, cholesterol concentrates, and purified proteins.
