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Defined Bioscience’s Ready-CEPT 1000X cocktail is a defined formulation designed for improved stem cell survival and growth during routine passaging, cryopreservation, and freeze-thaw recovery. Ready-CEPT is a single-vial cocktail of chroman-1 (a ROCK inhibitor), emricasan (a pan-caspase inhibitor), polyamines (putrescine, spermine, and spermidine; polycations for cell growth support), and trans-ISRIB (an ISR inhibitor). CEPT was originally developed at the National Institutes of Health (NIH), where it was found to improve single-cell cloning and sorting, as well as improved viability and cell health. These benefits were well-characterized in particular for genetically stable stem cell culture, with benefits in cell passaging, cryoprotection, organoid and embryoid body (EB) formation, and gene editing, with fewer apparent off-target effects compared to other similar products on the market (e.g. Y-27632).
Ready-CEPT is provided as a 1000X cocktail in a DMSO/water mixture, all using animal origin-free componentry.
Ready-CEPT 1000X is provided in liquid form in 10- and 200-µL volumes, sufficient for 10 and 200 mL of CEPT-supplemented medium, respectively. This supplement can be used with a wide range of formulations, including Defined Bioscience’s fully defined HiDef-B8 medium (Catalog # LSS-204).
Each lot of Ready-CEPT is used in combination with HiDef-B8 medium in performance testing in a culture assay evaluating using human iPSCs.
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With over 20 years of experience in the life science industry, we work hard to bring our customers the highest quality and most relevant products and services. We specialize in cell culture and diagnostic processed serum, cholesterol concentrates, and purified proteins.
