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Gelatin-based dissolvable microcarriers with Soft, Medium, and Hard stiffness grades, harvested by enzymatic dissolution instead of filtration.
X1 dissolvable microcarriers are made from a gelatin-based material designed to support attachment and expansion of adherent cells. Because the surface is biologically derived, it provides integrin-binding sites that many cell types recognize natively, without the need for a separate coating step.
X1 is removed through enzymatic digestion using TrypLE or Trypsin, typically 20 to 30 minutes depending on the stiffness variant, enabling cell harvest as a single-cell suspension without filtration or mechanical separation.
In evaluations using C2C12 myoblasts, X1 showed greater than 90% cell attachment within 24 hours, expansion of up to 30-fold over 7 days depending on seeding density, cell recovery approaching 100% following dissolution, and post-harvest viability exceeding 90%, with differentiation capacity maintained after expansion.
KEY FEATURES
APPLICATIONS
SPECIFICATIONS
| Material | Gelatin-based |
| Format | Freeze-dried powder, supplied by surface area (cm²) |
| Stiffness | Soft, Medium, Hard, customizable 3–100 kPa |
| Particle size | 100–150 µm, customizable |
| Dissolvability | Yes, TrypLE or Trypsin, 20–30 minutes |
| Chemical removal | Bleach or sodium hydroxide |
| Sterilization | Autoclave, heat, or gamma irradiation |
| Grade | Research, Food Grade, GMP-compliant options |
| Certificate of Analysis | Provided |
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